reaction monitoring (MRM), also known as selected reaction monitoring (SRM), and parallel
reaction monitoring (PRM) are targeted proteomics technologies aimed at quantifying up to
hundreds of proteins in the same
Multiple reaction monitoring
In an MRM workflow,
proteins are analyzed on a triple quadrupole mass spectrometer. The quadrupole
1 (Q1) is used as a mass filter to select a given precursor which is fragmented
in quadrupole 2 (Q2). In quadrupole 3 (Q3) a predefined number of product ions
from every precursor are analyzed. This enables highly sensitive protein
quantification over seven orders of magnitude.
Parallel reaction monitoring
PRM differs from MRM on the fact that all fragment ions instead of only selected ones are measured after
fragmentation of a selected precursor. For this reason, PRM is typically performed on Orbitrap or Time of Flight (ToF) analyzer.
Are MRM and PRM suitable to do absolute quantification?
Both technologies are suitable to perform absolute quantification by
using stable isotope heavy labeled reference peptides spiked into the sample at
SpectroDive is Biognosys' software solution to analyze MRM and PRM data. SpectroDive will assist you from
library and panel generation, MS method setup, to data analysis and results interpretation. To know more about
it, you can keep on reading here.
What are MRM and PRM?
Multiple reaction monitoring (MRM), also known as selected reaction monitoring (SRM), and parallel reaction monitoring (PRM) are targeted proteomics technologies aimed at quantifying up to hundreds of proteins in the same experiment. Multiple ...
What's the purpose of SpectroDive™?
SpectroDive has been developed to analyze targeted proteomics experiments, and it supports multiple reaction monitoring (MRM), parallel reaction monitoring (PRM), and SureQuant proteomics data. Since SpectroDive 11, it also supports technologies with ...
Which mass spectrometric instruments are supported by QuiC?
QuiC supports following MS instruments: • Thermo Scientific™ Orbitrap Exploris™ series (FAIMS 15 – 75 CV) • Thermo Scientific™ Orbitrap Eclipse™ Tribrid™ • Thermo Scientific™ Q Exactive™ Series • Thermo Scientific™ Orbitrap Fusion™ Series • Thermo ...
How to generate targeted panel in SpectroDive™?
In SpectroDive you can generate new targeted panels directly from a library. To do that you first need to have your library loaded into the Library Perspective in SpectroDive. We recommend generating your library with Pulsar, Biognosys' search ...
What is PlasmaDeepDive™ kit?
PlasmaDeepDive Reference Peptides enable absolute quantification of 100 human plasma proteins in a single measurement in scheduled MRM or PRM experiments, covering a dynamic range of six orders of magnitude, with high precision and reproducibility. ...